96 well plate
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[STEMCELLTechnologies™] [♪ 音乐声 ♪] [EasySep™ EasyPlate™磁极：使用96孔板进行简单快捷的细胞分选] 少量样本的高通量细胞分选 [Vicki Stronge博士 — 免疫学，产品经理]
在免疫检测研发和免疫学研究中经常被使用 通过EasyPlate™ EasySep™磁极可以在标准的96孔板上 简单快捷地分选未经标记的细胞 通过EasyPlate™分选的样品，每孔可 容纳多达2 x 10^7的小鼠细胞 或多达1 x 10^7的人细胞
短至25分钟内分选细胞 本视频将演示如何使用EasyPlate™ 分选未经标记的人B细胞 首先将25-200 μL PBMCs移入 未经组织培养处理的圆底96孔板 细胞悬液的浓度应为5 x 10^7 细胞/mL
向细胞悬液中加入EasySep™抗体混合物 并混合均匀 室温孵育10分钟 随后，短暂地震荡EasySep™磁珠 将磁珠加入细胞悬液中 室温孵育5分钟 加入EasySep™缓冲液，使每孔总容量达到250 μL 轻轻吹打，混匀细胞 将96孔板平稳地置于EasyPlate™磁极上，静置10分钟 被磁珠标记的细胞将被吸附于96孔板底部
分离出的B细胞即可 方便用于下游分析 欲在实验室中体验EasyPlate™ EasySep™磁极 可通过网站www.easysep.com申请免费产品演示 [ReadySepGo：短至25分钟的细胞分选] [STEMCELL Technologies™] [Scientist Helping Scientists™] [www.stemcell.com]
tags:
Cell isolation, cell separation, B cells, T cells, Monocytes, Natural killer cells, NK cells, immunomagnetic cell separation, immunomagnetic cell isolation, …
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